Hypoxia-induced animal modeling is used for the study of diseases of the cardiovascular system
1. Hypoxia induction method for animal modeling in the field of pulmonary hypertension
Principle: Simulating the hypoxic environment of the plateau, so that mice are in a state of hypoxia for a long time, resulting in pulmonary vasoconstriction and pulmonary vascular remodeling, which in turn causes pulmonary hypertension.
Common method: Place the mouse in a special hypoxic chamber, maintain the oxygen concentration in the chamber at 10%-12%, and continue hypoxia for 8-12 hours a day for 3-4 weeks. Alternatively, mice can be successfully modeled for pulmonary hypertension by living in an anoxic environment (e.g., a hypoxic chamber) for a long period of time with an oxygen concentration of around 10% for 4 to 6 weeks.
2. Myocardial ischemia-reperfusion injury:
Mice are placed in a hypoxic environment and then restored to normal oxygen supply, which can simulate the process of myocardial ischemia-reperfusion. This model is helpful to study the injury mechanism of cardiomyocytes during hypoxia and reoxygenation, oxidative stress response, and to find effective myocardial protective measures.
3. Heart failure:
Long-term hypoxia can lead to myocardial cell damage, heart remodeling, and then heart failure. The mouse heart failure model established by hypoxia induction method can be used to study the occurrence and development mechanism of heart failure, evaluate the changes of cardiac function, and develop new therapeutic drugs and interventions.
